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A theoretical study of the analytical solutions and behavior of some nonlinear differential equations using different control techniques
Author
الشهري، أحلام عبدالله علي
Supervisor
Dr. Yasser Salah Hamed Hassanein / Dr. Mohamed Sayed Abd Elkader
Category
Maths
Type
Master
Year
2017
Hit
0
Vibration, occurring in most machines, vehicles, structures, building and dynamic systems is undesirable, the reduction in performance which accompany vibrations and the dynamic stresses which may lead to fatigue and failure of the structure or machine. Vibration analysis of machines and structures is often a necessary prerequisite for controlling vibration. The vibrations of any dynamical system are needed to be controlled to minimize or eliminate the risk of damage or destruction. So many passive and active control techniques are used to eliminate or suppress the vibrations to minimum level. One of the important subjects expressed here is the dynamical systems, which are governed by different types of non-linear differential equations. The main object of this thesis is a mathematical and numerical study for the vibration behaviors in two different systems which are described by non-linear differential equations. The first system describe the active vibration control of a nonlinear two-degree-of-freedom system due to electromechanical system under external and parametric excitation. The second system study the nonlinear integral positive position feedback control of a cantilever beam system carrying an intermediate lumped mass by a three -degree-of-freedom with external excitation force. For each system, the investigation includes the analytical solutions applying the multiple scales perturbation technique (MSPT), determination of the resonance cases, which are classified into different categories. Some of resonance cases are confirmed numerically. The stability of each vibrating system and its steady state solutions are investigated by using both the frequency response equation and phase-plane technique. The numerical solutions are focused on both the effects of the different parameters and the behavior of the system at the selected resonance cases. Comparison between analytical and numerical solutions is obtained. Also all the numerical results are obtained using MAPLE and MATLAB algorisms. Some recommendations are given regarding the design of such system.
A thesis submitted in partial fulfillment of the requirements for the Master Degree of Science of Chemistry (Analytical Chemistry)
Author
العتيبي ، ريم سطام منير
Supervisor
د . إيمان سعد الزهراني - د . رشا عبداللطيف أحمد
Category
chemistry
Type
Master
Year
2018
Hit
0
This work has been studied fabrication of film composite membrane using macro-hydrogel method in presence of nanoparticles that was titanium dioxide (TiO2). The preparation of the film composite membranes was optimized by investigating different parameters such as the amount of the added chitosan, the amount of the added nanoparticles 0.03 g,0.05 g and 0.07g of (TiO2) the type of nanoparticles, and the thickness of the film membranes. The fabricated materials were characterized using different techniques such as scanning electron microscopy (SEM), ultraviolet spectroscopy (UV), energy dispersive X-ray spectroscopy (EDX) and swelling ratio. The prepared film composite membranes were used for degradation of harmful dyes (single dye and binary dyes). Different parameters such as pH (3,6,12) and amounts of oxidizing agent (250, 150, 25, 20 mg) of Potassium persulfate (K2S2O8) was optimized. In addition, the optimised conditions and film (chitosan (CS) + TiO2 (high concentration) membrane) were utilised for photodigradation of binary dyes methyl orange (MO), methyl blue (MB) from ground water and sewage water.
Algebraic connection with rough set theory
Author
الطويرقي، عبدالله عابد
Supervisor
Prof.Dr.Abd El-Monem Mohammed Kozae / Dr. SalehAyadOmran
Category
Maths
Type
Master
Year
2014
Hit
0
The main role of mathematics is to construct models for obtaining best solutions of real life problems. Set theories are models for general representations of collections under study. Rough set theory is an extension of conventional set theory that have great importance in applications. Rough set theory was proposed in 1982 by Z. Pawlak [19], is in a state of constant development. Its methodology is concerned with the classification and analysis of impreciseuncertain or incomplete information and knowledge is considered one of the first which did not depend on statistics in data analysis. This thesis consists of three chapters: Chapter One,is devoted to give an account on basic rough set concepts with examples. Section 1.1 Is given an introduction these concepts, the purpose of section 1.2 is to give an account on basic concepts of rough sets, section 1.3 is devoted to give for attribute redaction. Chapter Two, is to purpose of this chapter two is to explore some connections between Boolean algebra and rough set in general and with special interest to knowledge reduction of information systems , Section2.1is devoted to give a basic concept of Boolean algebra, the main goal ofSection 2.2 is to explain adetail the concept of attribute reduction and decision rule extraction by the aim using Boolean algebra . Chapter Three, The aim of chapter three is to study rough equality in Boolean algebra by using lower and upper approximation. Also, we construct the equality and the inclusion relations on the power set byusing topology and rough approximations section 3.1is contained on basic notions and examples of topological Boolean algebra. In section 3.2 some properties of rough equality in topological Boolean algebra is introduced.Section 3.3 is devoted to give rough ordering on power sets. Finally, section 3.4 is reserved for topological equality and inclusion relations.
Algebraic connections with rough set theory r
Author
الطويرقي، عبدالله عابد
Supervisor
Prof.Dr.Abd El-Monem Mohammed Kozae / Dr. SalehAyadOmran
Category
Maths
Type
Master
Year
2014
Hit
0
The main role of mathematics is to construct models for obtaining best solutions of real life problems. Set theories are models for general representations of collections under study. Rough set theory is an extension of conventional set theory that have great importance in applications. Rough set theory was proposed in 1982 by Z. Pawlak [19], is in a state of constant development. Its methodology is concerned with the classification and analysis of impreciseuncertain or incomplete information and knowledge is considered one of the first which did not depend on statistics in data analysis. This thesis consists of three chapters: Chapter One,is devoted to give an account on basic rough set concepts with examples. Section 1.1 Is given an introduction these concepts, the purpose of section 1.2 is to give an account on basic concepts of rough sets, section 1.3 is devoted to give for attribute redaction. Chapter Two, is to purpose of this chapter two is to explore some connections between Boolean algebra and rough set in general and with special interest to knowledge reduction of information systems , Section2.1is devoted to give a basic concept of Boolean algebra, the main goal ofSection 2.2 is to explain adetail the concept of attribute reduction and decision rule extraction by the aim using Boolean algebra . Chapter Three, The aim of chapter three is to study rough equality in Boolean algebra by using lower and upper approximation. Also, we construct the equality and the inclusion relations on the power set byusing topology and rough approximations section 3.1is contained on basic notions and examples of topological Boolean algebra. In section 3.2 some properties of rough equality in topological Boolean algebra is introduced.Section 3.3 is devoted to give rough ordering on power sets. Finally, section 3.4 is reserved for topological equality and inclusion relations.
Amelioration of Cadmium Effects with Tribulus terrestris in HEK Cell Line.
Author
المالكي ، وجدان مسفر محمد
Supervisor
Prof. Dr. Metwally M. Montaser
Category
Biology
Type
Master
Year
2019
Hit
0
BACKGROUND: cadmium is among the toxic and hazardous metals widely dispersed in the environment at high levels; it is known to be toxic for the kidney. Tribulus terrestris L. (TT) plants in Saudi Arabia. It has been used as a protective herb for kidney disorders. The present research aims to study the possible ameliorative potential of T. terrestris extract on Cd- induced effects in human embryonic kidney (HEK 293) cell line. METHODS: we have studied the cell viability and IC₅₀ by using SRB. Then, cells were divided into six groups of triplicates treated for 12 h and 24 h as follows: group 1, normal (untreated) control; group 2, cadmium chloride (CdCl₂) treated for 12 h; group 3, T. terrestris extract-treated for 12 h; group 4, (amelioration) CdCl₂ for 12 h proceeded by T. terrestris extract for 12 h ; group 5 (mix), CdCl₂ plus T. terrestris extract-treated for 12 h; group 6, cadmium chloride (CdCl₂) treated for 24 h. We investigated the ameliorative potential at the cellular and molecular levels; apoptosis by using acridine orange/ethidium bromide staining, Annexin V/PI staining, cell kinetics, and induction response of some detox (SOD1, CYP1A1, and UGT) and anti-apoptotic (Bcl-2) genes. RESULTS: Cd decreased HEK 293 viability in a dose-dependent manner with with an IC₅₀ value of 15.06 g/ml Nuclear morphological analysis revealed that Cd-induced cell necrosis. It also positively induced detox genes and reduced Bcl-2. The inoculation of T. terrestris extract after CdCl₂ treatment reduced most of the Cd-induced toxicity. However, mixing or contaminating the extract with Cadmium rendered the ameliorative potential of the extract. CONCLUSION: the ameliorative action of the plant could be due to the presence of antioxidants and tribulin, which is the major ingredient in the plant. Our study recommends the use T. terrestris as a Cd-toxicity recovery herb. However, the current study needs to be confirmed at further research levels such as microarray, and the application on experimental animals (in vivo
An Investigation into The Effect of Nano-Coating on The Corrosion Inhibition of Carbon Steel in The Crude Oil Mediums
Author
العصيمي، منيرة ناصر ضيف الله
Supervisor
Dr. Hala Abo-Deif Abo- Zeid / Dr. Amal Ahmad AL-Talhi
Category
chemistry
Type
Master
Year
2016
Hit
0
Pipelines are major part of any oil and petrochemical industry. In this study, the corrosion of oil and gas pipelines made from carbon steel is controlled by Protective coatings. An attempt has been made to develop Nano- Emulsion coatings based on olive oil , paraffin oil and silicon oil (oil phase) as a replacement of organic solvent based coatings system in surface finishing industry for environmental protection, public health and safety. In this research, oil-in-water (O/W) Nano-emulsions were prepared by means of a low-energy method .The O/W Nano-emulsion was prepared using three oils (olive oil , paraffin oil and silicon oil) ,tween 20 and tween 80 as surfactant and liquids phase( green tea extract ,fenugreek extract and Garlic extract) . It was formulated for various concentrations of oils and surfactants. The stable Nano-emulsion ratio was taken for the study. Eco-friendly coatings (Nano-emulsion +ZnO + poly(ethylene glycol) on the carbon steel surface were done using electrophoretic deposition method (EPD). ZnO plays an important role in the chemical industry because of its stable physical and chemical properties and high oxidation activity . The electrophoretic deposition (EPD) of eco-friendly Nano-Coatings on carbon steel substrates was investigated by FTIR, scanning electron microscopy, potentiodynamic polarization measurements, and electrochemical impedance spectroscopy (EIS). Eco-friendly coatings showed very good inhibition efficiency (IE) in sulphuric acid solution, hydrocloric acid and formation water. The method enabled the formation of adherent, protective and uniform coatings. Comparing 24 Coating with one another based on the impedance and potentiodynamic polarization value to determine the best Coating in various test solutions. In HCl solution : the coating that contain paraffin oil with Garlic and tween 80 shows higher values of corrosion resistance and In H2SO4 and in formation water: , The coating containing olive oil with garlic and tween 80 shows higher values of corrosion resistance.
Analytic and numerical solutions for some nonlinear partial differential equations
Author
الثبيتي، علي عبدالله
Supervisor
Dr. Khaled Ahmad Gepreel / Dr. Taher Abd Alhameed Nofal
Category
Maths
Type
Master
Year
2018
Hit
0
The main objective of this thesis is to obtain the exact solutions and approximate solutions for some nonlinear partial differential equations, nonlinear partial fractional differential equation and nonlinear differential difference equations in mathematical physics. In chapter 1, we state some methods for finding the approximate and analytic exact solutions for nonlinear partial differential equations. In the second chapter, we use the modified rational Jacobi elliptic functions method to find some exact solutions for nonlinear differential difference equations in mathematical physics namely the lattice equation, the discrete nonlinear Schrodinger equation with a saturable nonlinearity, the discrete nonlinear Klein Gordon equation and the quintic discrete nonlinear Schrodinger equation. Some new types of the Jacobi elliptic solutions are obtained for some nonlinear differential difference equations in mathematical physics. The results of this chapter have been published in [1]. In the third chapter, we discussed the exact solutions for nonlinear PDE's in mathematical physics by using some new direct methods namely the extended coupled sub-equations expansion method and the modified simple equation method. The first part of this chapter, we have used the extended coupled sub-equations expansion method to get some series of double soliton-like solutions, double triangular function solutions and complexiton soliton solutions for some nonlinear partial differential equations via the (2+1)- dimensional breaking soliton equations and the (2+1)- dimensional Nizhnik-Novikov- Vesselov equations. We obtain many types of double soliton solutions as various combination of trigonometric periodic function and hyperbolic function, various combination of trigonometric periodic function and rational function, various combination of hyperbolic function and rational function. The results of this subsection have been published in [2]. The second part of this chapter, we have used the modified simple equation method to construct the exact traveling wave solutions for some nonlinear PDE's in mathematical physics namely the coupled Higgs equations, the coupled Maccaris equations and the Drinfeld–Sokolov system. Based on this formulation, solitary solutions can be easily obtained by using the proposed method. The proposed method is direct and more powerful than the other methods. The results of the second part of this chapter have been submitted for published in [3]. In the fourth chapter, we have studied some exact and approximate solutions for nonlinear partial fractional differential equations. The first part of this chapter, we implement relatively analytical techniques such as the homotopy perturbation method and homotopy analysis method to solve nonlinear partial fractional differential Zakharov-Kuznetsov equations. The fractional derivatives are described in the Caputo sense. We have compared between the approximate solutions which obtained by the homotopy perturbation method and the approximate solutions which obtained by homotopy analysis method. Also we make the Figures to compare between the approximate solutions. We compare between the approximate solutions and the exact solutions for the partial fractional differential equations when , , 1. The results of the first part of this chapter have been submitted for published [4] . The second part of this chapter according to the local fractional derivative and the fractional sub-equation method, we have obtained some of the exact solutions for nonlinear partial fractional differential equations namely the space–time fractional coupled Sakharov- Kuznetsov (Z-K) equations, the space–time fractional nonlinear coupled KdV equations and the space–time fractional Hirota Satsuma KdV system. As a result, three families of exact analytical solutions are obtained. The results of the second part of this chapter have been submitted for published in [5].
Analytic and numerical solutions for some nonlinear partial differential equations
Author
الثبيتي، علي عبدالله
Supervisor
Dr. Khaled Ahmad Gepreel / Dr. Taher Abd Alhameed Nofal
Category
Maths
Type
Master
Year
2012
Hit
0
The main objective of this thesis is to obtain the exact solutions and approximate solutions for some nonlinear partial differential equations, nonlinear partial fractional differential equation and nonlinear differential difference equations in mathematical physics. In chapter 1, we state some methods for finding the approximate and analytic exact solutions for nonlinear partial differential equations. In the second chapter, we use the modified rational Jacobi elliptic functions method to find some exact solutions for nonlinear differential difference equations in mathematical physics namely the lattice equation, the discrete nonlinear Schrodinger equation with a saturable nonlinearity, the discrete nonlinear Klein Gordon equation and the quintic discrete nonlinear Schrodinger equation. Some new types of the Jacobi elliptic solutions are obtained for some nonlinear differential difference equations in mathematical physics. The results of this chapter have been published in [1]. In the third chapter, we discussed the exact solutions for nonlinear PDE's in mathematical physics by using some new direct methods namely the extended coupled sub-equations expansion method and the modified simple equation method. The first part of this chapter, we have used the extended coupled sub-equations expansion method to get some series of double soliton-like solutions, double triangular function solutions and complexiton soliton solutions for some nonlinear partial differential equations via the (2+1)- dimensional breaking soliton equations and the (2+1)- dimensional Nizhnik-Novikov- Vesselov equations. We obtain many types of double soliton solutions as various combination of trigonometric periodic vi function and hyperbolic function, various combination of trigonometric periodic function and rational function, various combination of hyperbolic function and rational function. The results of this subsection have been published in [2]. The second part of this chapter, we have used the modified simple equation method to construct the exact traveling wave solutions for some nonlinear PDE's in mathematical physics namely the coupled Higgs equations, the coupled Maccaris equations and the Drinfeld–Sokolov system. Based on this formulation, solitary solutions can be easily obtained by using the proposed method. The proposed method is direct and more powerful than the other methods. The results of the second part of this chapter have been submitted for published in [3]. In the fourth chapter, we have studied some exact and approximate solutions for nonlinear partial fractional differential equations. The first part of this chapter, we implement relatively analytical techniques such as the homotopy perturbation method and homotopy analysis method to solve nonlinear partial fractional differential Zakharov-Kuznetsov equations. The fractional derivatives are described in the Caputo sense. We have compared between the approximate solutions which obtained by the homotopy perturbation method and the approximate solutions which obtained by homotopy analysis method. Also we make the Figures to compare between the approximate solutions. We compare between the approximate solutions and the exact solutions for the partial fractional differential equations when , , 1. The results of the first part of this chapter have been submitted for published [4] . The second part of this chapter according to the local fractional derivative and the fractional sub-equation method, we have obtained some of the exact solutions for nonlinear partial fractional differential equations namely the space–time fractional vi coupled Sakharov- Kuznetsov (Z-K) equations, the space–time fractional nonlinear coupled KdV equations and the space–time fractional Hirota Satsuma KdV system. As a result, three families of exact analytical solutions are obtained. The results of the second part of this chapter have been submitted for published in [5].
Analytic Methods For Nonlinear Evolution Equations In Mathematical Physics
Author
السيالي، نهال سعد معيوض
Supervisor
Dr. Khaled A. Gepreel / Dr. Taher A. Nofal
Category
Maths
Type
Master
Year
2017
Hit
0
The main object of this thesis is to obtain the exact solutions for nonlinear partial differential equations and approximate solutions for the nonlinear partial fractional differential equations. In this thesis we use a different methods to solve the nonlinear partial differential equation such as extended trail equation method, multiple expansion function method, extended (G /G) expansion function method. Also, we use the reduced differential transform method for discuss the approximate solutions for the nonlinear partial fractional differential equations. We compare between the approximate solutions and exact solution for nonlinear partial fractional differential equations. We get the reduced differential transform method is rapidly convergence to exact solution. We make some figures to illustrate the comparison between exact and approximate solutions.
Analytical and Numerical Solutions of Constrained Parabolic Optimal Control Problems
Author
الثبيتي، فهد باخت محيل
Supervisor
Dr.Moustafa Mohammad Moustafa / Dr.Mohamed A. El-Sayed
Category
Maths
Type
Master
Year
2018
Hit
0
Recent years, there are wide applications of Optimal control like in biology, engineering, aerospace, physics, chemistry, economics and other areas of science and has received considerable attention of researchers. In this thesis, a proposed system of optimal control problem with respect to a parabolic equation is obtained. The existence and uniqueness of the system is introduced. In this way, the uniqueness theorem for solving parabolic optimal control problem is presented. Therefore, the theorem for the sufficient differentiability conditions has been proved. By using the exterior penalty function method, the constrained problem is converted to a new unconstrained problem. The variation of the functional and its gradient is presented. Lipchitz continuity of the gradient of the cost functional is derived. The common techniques of constructing the gradient of the cost functional using the solving of the adjoint problem is investigated a computational approach based on an exterior penalty function with modified partial quadratic interpolation method is given for solving a class of a constrained parabolic optimal boundary control problem which the final profile can be a nonlinear or linear function. Also, it is applied to solving the inverse heat conduction problem. The outlined of the algorithm for solving the optimal control problem and its convergence is given. Numerical results for constrained optimal control parabolic problems are obtained. All results are carried out using ForTran Language.
Analytical and Numerical Study for Some New Problems in Dynamical Systems Described by Some Differential Equations
Author
الأسمري ، فاطمة سعيد عبود
Supervisor
د . عماد الدين محمود
Category
Maths
Type
Master
Year
2017
Hit
1
In this thesis, we introduce and analyze a novel chaotic complex nonlinear system. The dynamics of this system is rich in the sense that our system exhibit chaotic attractors as well as periodic, quasi periodic solutions and solutions that approach fixed points. The stability analysis of the trivial fixed pointis stated. Numerically the range of parameters values of this system at which chaotic attractors exist is calculated. Lyapunov exponents are computed to classify the dynamics of this system. Symmetry, invariance and dissipation are discussed The principal target of this work is to introduce and examine a novel kind of complex synchronization. This sort may be called complex anti lag synchronization (CALS). There are surprising properties of CALS that do not exist in the writing, for example, (i) The CALS contains two sorts of synchronizations (anti-lag synchronization ALS and lag synchronization LS). (ii( In CALS the attractors of the main and slave systems are moving opposite or similar to each other with time lag. (iii) The state variable of the main system synchronizes with a different state variable of the slave system. We introduce and examine another kind of complex synchronization. We call this type complex modified projective lag synchronization (CMPLS). CMPLS, which is a more general case of synchronizations, is rarely studied or not mentioned up to date. In order to show this synchronizatio n, we study it to a system with certain and uncertain parameters of two chaotic complex n-dimensional flows possessing chaotic attractors with varying totally or partially in nonlinear terms The thesis consists of five chapters: Chapter one: In this chapter we show the preliminaries of nonlinear dynamical systems. We give a review of some basic concepts and definitions of nonlinear dynamical systems such as fixed points, Lyapunov functions, attractors, Lyapunov exponents, chaotic systems, hyperchaotic systems, secure communications, chaos and synchronization Chapter two: This chapter introduces and analyzes a new chaotic complex nonlinear system. This system is 6 dimensional systems of real first order autonomous differential equations. The dynamics of this system are contemplated. The Lyapunov dimension of the chaotic attractors of these systems is calculated. We presented different types of our system. Also, we introduced a definition of a new type of synchronization and studied it on our system. An approach proposed to analyze CALS of the complex system in light of Lyapunov function . Chapter three: The aim of this chapter is to investigate CALS with uncertain parameters. A definition of CALS with uncertain parameters is introduced for two identical chaotic complex nonlinear models. Based on Lyapunov function a scheme is designed to achieve CALS of chaotic attractors of these systems. The effectiveness of the obtained results is illustrated by a simulation example. The chaos synchronization results were employed to develop a simple application in secure communication. Chapter four: In this chapter, we present and investigate the definition of a new type of complex synchronization. We may call this type "complex modified projective lag synchronization (CMPLS)". We design schemes to achieve CMPLS of two chaotic complex systems with varying in nonlinear terms and with certain and uncertain parameters. We applied these schemes to study CMPLS on two chaotic complex nonlinear systems with certain and uncertain parameters. All the analytical results are verified by numerical simulation of our examples Chapter five: In this chapter, we conclude our main results and suggest some future works.
Anatomical Studies on the Cranial Nerves and Ganglia of the Head Region of the Lizard Uromastyx aegyptia microlepis
Author
الوقداني ، إيمان أحمد سند
Supervisor
Dr. Nahed A. Hussien - Prof. Dr. Ahmed E. Dakrory
Category
Biology
Type
Master
Year
2015
Hit
0
Anatomical Studies on the Cranial Nerves of Oreochromis niloticus (Ostiechthyes – Cichlidae)
Author
المالكي، غرسه جابر أحمد
Supervisor
Prof. Dr. Ahmed Emam Ahmed Dakrory / Dr. Thrwat Ghanem Abdelkader
Category
Biology
Type
Master
Year
2017
Hit
0
The organization of the roots, ganglia and the peripheral distribution of the cranial nerves in the fully formed embryos of Oreochromis niloticus are examined in the serial transverse sections. The analysis of the fibres carried by these nerves is studied. The results of this study demonstrated that the olfactory peduncle is absent. There is a complete optic deccussation. The ciliary ganglion is well formed and has one ciliary nerve. The nervus trigeminus has a single extracranial Gasserian ganglion. The intracranial geniculate ganglion of the facial nerve is partially fused with the ganglion of the anteroventral lateral line nerve. There is only one ramus palatinus that fuses with ramus maxillaries of the nervus trigeminus. The truncus hyomandibularis separates into three rami; opercularis, hyoideus and mandibularis. The nervus octavus runs ganglionated and has six branches and has no branch of macula neglecta. The nervus glossopharyngeus has one root, and has its own foramen and one extracranial petrosal ganglion. This nerve has three rami; ramus pretrematicus, ramus posttrematicus and ramus pharyngeus. The nervus vagus arises separately by two roots.It leaves the cranial cavity together with the posterior lateral line nerve through the jugular foramen. It has three branchial and one visceral trunks; each has its own ganglion. Each branchial vagal trunk gives rise to four rami; pharyngeus, lower pretrematic, upper pretrematic and posttrematic. There is an intracranial dorsal vagal ramus. There are two pairs of lateral line nerves; anterior and posterior and the middle lateral line nerve is lacking. The anterior lateral nerve divides into anterodorsal and anteroventral lateral line nerve. The anterodorsal lateal line nerve gives rise to three rami; ophthalmicus superficialis lateralis, buccalis and recurrence lateralis. The anteroventral line nerve fuses completely with the hyomandibular trunk of the nervus facialis.
Antibacterial activity of silver nano-particles against some species of family Enterobacteriaceae isolated from different sources at Taif Kingdom of Saudi Arabia
Author
النمري ، أحلام حسن
Supervisor
Prof.Dr. Moataz Hassan Ahmed EL-Sehrawy -Dr. Abeer Ahmed Abu Zaid
Category
Biology
Type
Master
Year
2016
Hit
1
The present study aimed to Biosynthesis of silver nano particles by using some plant extracts , of Elettaria cardamomum , Laurus nobilis and Rosmarinus officinalis and assessment their antibacterial activity and cytotoxicity. The presence of silver nano particles was initially confirmed by colored developed from colorless to dark brown within the first 10 min , then by UV- VIS spectrometer and Transmission electron microscope (TEM). Laura silver nano particles were showed the sharp peak around 411 nm with high density. TEM images exhibited the nano particles synthesized by each plant extract were relatively spherical in shape and ranged size (15-50 nm). Twenty two bacterial strains (A01-A22) were isolated from juices, fruits and vegetables salad in restaurants at governorate Taif Kingdom of Saudi Arabia, then characterized by morphology, biochemical reactions and confirmed by API 20 E system. Further the strains were identified by partial 16SrRNA gene sequencing and phylogenetic tree analysis. All isolates were identified by 95-99% identity, except strain A13 was not complete. Eleven isolates out of twenty two were identified as K. pneumnoniae, nine were K. quasipneumnoniae, and only one named by K. oxytoca. Ten bacterial strains (A23-A32) were isolated from urine in Maternity and Children Hospital at Makkah City-Kingdom of Saudi Arabia, then characterized by morphology, biochemical reactions and confirmed by Microbact assay. All isolates were identified with type strain of Escherichia coli. In addition, antibiotic resistance profiles of isolated strains were determined. Thirty two isolates were resistance to Penicillin G and Erythromycin, except Escherachia coli 24A. The highest number of antibiotics resistance were recorded by E.coli A32 and A30, which displayed multidrug resistance against 7, 8 types of antibiotics used in this study respectively . In conclusion, the antibacterial activity of each plant extract as aqueous plant extract, plant extract with silver nano particles, and their concentrations as well as chemical silver nano particles were evaluated against 32 isolates. The highest significant degree of growth inhibition at concentration (9:1) which contain 1 Mm from AgNO3 of Biosynthesis Cardamom Nano Silver Particles (BCNSP) was showed against all bacterial strains (26-37mm), except strain NO. A08, A20 and A24 were about (11mm). The correlation between BCNSP and viability of VERO cell line is negative and highly significant, where “r” value = -0.9029, where at 3.125µg/ml concentration BCNSP was showed 100% viability of VERO cell line .This suggests that silver nano particles from cardamom extract have great potential to act a safe alternative to antibiotics to fight the challenges of drug resistance. In addition, the applications includes also used in food industry as disinfection by adding to soap or sanitizer, for cleaning utensils and treatment waste , storage water. These results urge the testing of these green nano particles in vivo.
Antibacterial Effect of Silver Nanoparticles against Methicillin- Resistant Staphylococcus aureus Isolated from Different Sources at Taif Governorate
Author
نزار ، فريبا أمان الله حق
Supervisor
Prof. Dr. Nashwa abdelsalam ezzeldeen - Dr. Abeer Ahmed Abu Zaid
Category
Biology
Type
Master
Year
2016
Hit
0
In this study, fifty-one samples of milk collected from various sources, and to study the spread of the bacteria Staphylococcus aureus rate, which was the most important causes of mastitis. From the results, bacterial strains (F1-F14) were isolated from milk as follows: 9 camel, 3 goat and 1 both cow and sheep. The isolates characterized by morphology, biochemical reaction and identified by molecular methods. In vitro the susceptibility of S. aureus strains were done against 9 antibiotics. The results showed that all milk strains (14) were sensitive to ciprofloxacin, followed by tetracycline 64.3 %. While, the resistant strains (14) were against amikacin and oxacillin 100% followed by gentamicin 92.9 %. Antibacterial activity of aqueous each plant extract (cardamom, rosemary and laura) were done against 14 strains of S. aureus. The results were did not record any effect, while the chemical silver nanoparticle (AgNPs) gave different antibacterial activity against all strains. In addition, the synthesized plant AgNPs showed significant antibacterial effect against all bacterial strains. Using of UV Vis Spectroscopy and Transmission Electron Microscope (TEM) techniques to characterized the green synthesized nanoparticles. In vitro, cytotoxic effects of AgNPs were screened against Vero cells. The chemical, cardamom, rosemary and laura silver nanoparticles were able to reduce viability of the Vero cells in a dose-dependent manner.